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- Title
The Vinca minor genome highlights conserved evolutionary traits in monoterpene indole alkaloid synthesis.
- Authors
Stander, Emily Amor; Cuello, Clément; Birer-Williams, Caroline; Kulagina, Natalja; Jansen, Hans J.; Carqueijeiro, Ines; Méteignier, Louis-Valentin; Vergès, Valentin; Oudin, Audrey; Papon, Nicolas; Dirks, Ron P.; Jensen, Michael Krogh; O'Connor, Sarah Ellen; de Bernonville, Thomas Dugé; Besseau, Sébastien; Courdavault, Vincent
- Abstract
Vinca minor, also known as the lesser periwinkle, is a well-known species from the Apocynaceae, native to central and southern Europe. This plant synthesizes monoterpene indole alkaloids, which are a class of specialized metabolites displaying a wide range of bioactive- and pharmacologically important properties. Within the almost 50 monoterpene indole alkaloids it produces, V. minor mainly accumulates vincamine, which is commercially used as a nootropic. Using a combination of Oxford Nanopore Technologies long read- and Illumina shortread sequencing, a 679,098Mb V. minor genome was assembled into 296 scaffolds with an N50 scaffold length of 6Mb, and encoding 29,624 genes. These genes were functionally annotated and used in a comparative genomic analysis to establish gene families and to investigate gene family expansion and contraction across the phylogenetic tree. Furthermore, homology-based monoterpene indole alkaloid gene predictions together with a metabolic analysis across 4 different V. minor tissue types guided the identification of candidate monoterpene indole alkaloid genes. These candidates were finally used to identify monoterpene indole alkaloid gene clusters, which combined with synteny analysis allowed for the discovery of a functionally validated vincadifformine-16-hydroxylase, reinforcing the potential of this dataset for monoterpene indole alkaloids gene discovery. It is expected that access to these resources will facilitate the elucidation of unknown monoterpene indole alkaloid biosynthetic routes with the potential of transferring these pathways to heterologous expression systems for large-scale monoterpene indole alkaloid production.
- Subjects
EUROPE; INDOLE alkaloids; GENOMICS; GENE families; GENOMES; GENE clusters; INDOLE; ALKALOIDS
- Publication
G3: Genes | Genomes | Genetics, 2022, Vol 12, Issue 12, p1
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1093/g3journal/jkac268