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- Title
Expression, Polymorphism, and Potential Functional Sites of the BMPR1A Gene in the Sheep Horn.
- Authors
Zhang, Guoqing; Chu, Mingxing; Yang, Hao; Li, Hao; Shi, Jianxin; Feng, Pingjie; Wang, Shoufeng; Pan, Zhangyuan
- Abstract
Simple Summary: This study investigated the relationship between the BMPR1A gene and horn type in sheep. The study found that the expression of the BMPR1A gene was significantly higher in the normal horn sheep compared to scurred sheep. This trend was observed in multiple sheep breeds. It was also discovered that there is high expression in the skin of three species: cattle, sheep, and pigs. Importantly, it was found that certain nucleotides in the BMPR1A gene are likely to be crucial in horn size and horn type. Sheep horns are composed of bone and sheaths, and the BMPR1A gene is required for cartilage and osteogenic differentiation. Therefore, the BMPR1A gene may have a function related to the sheep horn, but its relationship with the sheep horn remains unclear. In this study, we first utilized RNA sequencing (RNA-seq) data to investigate the expression of the BMPR1A gene in different tissues and breeds of sheep. Second, whole-genome sequencing (WGS) data were used to explore the functional sites of the BMPR1A gene. Lastly, the allele-specific expression of the BMPR1A gene was explored. Our results indicate that BMPR1A gene expression is significantly higher in the normal horn groups than in the scurred groups. Importantly, this trend is consistent across several sheep breeds. Therefore, this finding suggests that the BMPR1A gene may be related to horn type. A total of 43 Single-Nucleotide Polymorphisms (SNPs) (F-statistics > 0.15) and 10 allele-specific expressions (ASEs) exhibited difference between the large and small horn populations. It is probable that these sites significantly impact the size of sheep horns. Compared to other polled species, we discovered ten amino acid sites that could influence horn presence. By combining RNA-seq and WGS functional loci results, we identified a functional site at position 40574836 on chromosome 25 that is both an SNP and exhibits allele-specific expression. In conclusion, we demonstrated that the BMPR1A gene is associated with horn type and identified some important functional sites which can be used as molecular markers in the breeding of sheep horns.
- Subjects
GENE expression; SHEEP breeds; SHEEP; WHOLE genome sequencing; SHEEP breeding; GENES
- Publication
Genes, 2024, Vol 15, Issue 3, p376
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes15030376