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- Title
Human Tissue Plasminogen Activator Expression in Escherichia coli using Cytoplasmic and Periplasmic Cumulative Power.
- Authors
Majidzadeh, Keivan; Mahboudi, Fereidoun; Hemayatkar, Mahdi; Davami, Fatemeh; Barkhordary, Farzaneh; Adeli, Ahmad; Soleimani, Mohammad; Davoudi, Noushin; Khalaj, Vahid
- Abstract
Tissue plasminogen activator (tPA) is a serine protease, which is composed of five distinct structural domains with 17 disulfide bonds, representing a model of high-disulfide proteins in human body. One of the most important limitations for high yield heterologous protein production in Escherichia coil (E. coli) is the expression of complex proteins with multiple disulfide bridges. In this study the combination of two distinct strategies, manipulated cytoplasm and native periplasm, was applied to produce the functional full length tPA enzyme in E. coli. Using a PeIB signal peptide sequence at 5′ site of tPA gene, the expression cassette was prepared and subsequently was transformed into a strain with manipulated oxidizing cytoplasm. Then the induction was made to express the protein of interest. The SDS-PAGE analysis and gelatin hydrolysis confirmed the successful expression of functional tPA. The results of this study showed that complex proteins can be produced in E. coli using the cumulative power of both cytoplasm and periplasm.
- Subjects
TISSUE plasminogen activator; SERINE proteinases; ESCHERICHIA coli; CYTOPLASM; DIETARY proteins; THERAPEUTICS
- Publication
Avicenna Journal of Medical Biotechnology, 2010, Vol 2, Issue 3, p131
- ISSN
2008-2835
- Publication type
Article