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- Title
Bio-Oss acts on Stem cells derived from Peripheral Blood.
- Authors
Sollazzo, Vincenzo; Palmieri, Annalisa; Scapoli, Luca; Martinelli, Marcella; Girardi, Ambra; Alviano, Francesco; Pellati, Agnese; Perrotti, Vittoria; Carinci, Francesco
- Abstract
Objectives:This study aims to study how Bio-Oss® can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells markers using real time Reverse Transcription-Polymerase Chain Reaction. Methods: PB-hMSCs stem preparations were obtained for gradient centrifugation from peripheral blood of healthy anonymous volunteers, using the Acuspin System-Histopaque 1077. The samples were then cultured for 7 days for RNA processing, and the expression was quantified using real time PCR. Results: Bio-Oss® caused an induction of osteoblast transcriptional factor like RUNX2 and of bone related genes; SPP1 and FOSL1. In contrast, the expression of ENG was significantly decreased in stem cells treated with Bio-Oss® with respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells. Conclusion: The results obtained can be relevant to enhance the understanding of the molecular mechanism of bone regeneration and can act as a model for comparing other materials with similar clinical effects.
- Subjects
POLYMERASE chain reaction; STEM cells; REGENERATION (Biology); DNA polymerases; GENE amplification; GENE expression; BONE regeneration; BONE remodeling; CLINICAL medicine
- Publication
Oman Medical Journal, 2010, Vol 25, Issue 1, p1
- ISSN
1999-768X
- Publication type
Article
- DOI
10.5001/omj.2010.7