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- Title
Exploring microbial N<sub>2</sub>O reduction: a continuous enrichment in nitrogen free medium.
- Authors
Conthe, Monica; Kuenen, J. Gijs; Kleerebezem, Robbert; van Loosdrecht, Mark C. M.
- Abstract
Summary: N2O is a potent greenhouse gas, but also a potent electron acceptor. In search of thermodynamically favourable – yet undescribed – metabolic pathways involving N2O reduction, we set up a continuous microbial enrichment, inoculated with activated sludge, fed with N2O as the sole electron acceptor and acetate as an electron donor. A nitrogen‐free mineral medium was used with the intention of creating a selective pressure towards organisms that would use N2O directly as source of nitrogen for cell synthesis. Instead, we obtained a culture dominated by microorganisms of the <italic>Rhodocyclaceae</italic> family growing by N2O reduction to N2 coupled to N2 fixation. Biomass yields of this culture were 40% lower than those of a previously reported culture grown under comparable conditions but with an NH 4 +‐amended medium, as expected from the extra energy expense of N2 fixation. Interestingly, we found no significant difference in yields whether N2O or acetate was the growth‐limiting substrate in the chemostat in contrast to the study with NH 4 +‐amended medium, in which biomass yields were roughly 30% lower during acetate limiting conditions.
- Subjects
NITROGEN fixation; NITROGEN reduction; MICROBIAL cultures; MICROBIAL inoculants; ELECTROPHILES; CHEMOSTAT
- Publication
Environmental Microbiology Reports, 2018, Vol 10, Issue 1, p102
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.12615