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- Title
A novel W olbachia strain from the rice moth C orcyra cephalonica induces reproductive incompatibility in the whitefly B emisia tabaci: sequence typing combined with phenotypic evidence.
- Authors
Hu, Hong-Yan; Li, Zheng-Xi
- Abstract
W olbachia are a group of maternally inherited bacteria frequently found in arthropods and filarial nematodes. They have recently attracted attention for their ecological roles in manipulating host reproduction, their potential use in biological control of pest insects and medical significance. Classification of W olbachia strains is currently solely based on molecular methods. However, the strains even with identical sequence types may induce different host phenotypes. Here we isolated a W olbachia strain from the rice moth C orcyra cephalonica (designated as w Ccep_ B_ BJ), which was shown to share multilocus sequence typing and W olbachia surface protein hypervariable region profiles with a cytoplasmic incompatibility ( CI)-inducing strain in supergroup B, but the phenotype w Ccep_ B_ BJ may induce needs to be determined. We thus transinfected it into the whitefly B emisia tabaci harbouring an A- W olbachia through nymphal microinjection. Fluorescent in situ hybridization demonstrated that w Ccep_ B_ BJ was successfully transinfected into B. tabaci and transmitted to offspring through host eggs. Reciprocal cross showed that w Ccep_ B_ BJ induced a strong bidirectional CI in the transinfected host without imposing a significant cost on female fecundity. Our results suggest that w Ccep_ B_ BJ may be a promising strain for biocontrol of B. tabaci, an important agricultural pest insect.
- Subjects
WOLBACHIA; RICE moth; SWEETPOTATO whitefly; BIOLOGICAL control of insects; SEQUENCE analysis
- Publication
Environmental Microbiology Reports, 2015, Vol 7, Issue 3, p508
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.12279