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- Title
Domain-level identification and quantification of relative prokaryotic cell abundance in microbial communities by Micro-FTIR spectroscopy.
- Authors
Igisu, Motoko; Takai, Ken; Ueno, Yuichiro; Nishizawa, Manabu; Nunoura, Takuro; Hirai, Miho; Kaneko, Masanori; Naraoka, Hiroshi; Shimojima, Mie; Hori, Koichi; Nakashima, Satoru; Ohta, Hiroyuki; Maruyama, Shigenori; Isozaki, Yukio
- Abstract
Summary Domain-level identification of microbial cells or cell-like structures is crucial for investigating natural microbial communities and their ecological significance. By using micro-Fourier transform infrared (micro-FTIR) spectroscopy, we established a technical basis for the domain-level diagnosis and quantification of prokaryotic cell abundance in natural microbial communities. Various prokaryotic cultures (12 species of bacteria and 10 of archaea) were examined using micro-FTIR spectroscopic analysis. The aliphatic CH3/CH2 absorbance ratios ( R3/2) showed domain-specific signatures, possibly reflecting distinctive cellular lipid compositions. The signatures were preserved even after chemical cell fixation (formaldehyde) and nucleic acid staining (DAPI) processes - techniques that are essential in studying microbial ecology. The micro-FTIR technique was successfully applied for quantification of the bacteria/archaea abundance ratio in an active microbial mat community in a subsurface hot aquifer stream. We conclude that the micro-FTIR R3/2 measurement is both fast and effective for domain-level diagnosis and quantification of first-order prokaryotic community structures.
- Subjects
PROKARYOTES; MICROBIAL cell cycle; MICROBIAL ecology; BACTERIA; NUCLEIC acids
- Publication
Environmental Microbiology Reports, 2012, Vol 4, Issue 1, p42
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/j.1758-2229.2011.00277.x