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- Title
Dissemination of microbiota between wounds and the beds of patients with pressure injuries: a cross-sectional study.
- Authors
Kunimitsu, M.; Nakagami, G.; Kitamura, A.; Minematsu, T.; Mugita, Y.; Ogai, K.; Sugama, J.; Aoki, M.; Takada, C.; Sanada, H.
- Abstract
Aim Wound infection is a life-threatening complication of pressure injuries (PIs) and is not yet completely preventable. This study aims to explore the dissemination of microbiota between PIs and hospital beds using a culture-independent methodology. This serves as the first step towards developing a new intervention to prevent wound infection. Methods A cross-sectional study was conducted at a longterm care hospital on patients aged >65 years with PIs. The microbiota of wounds, skin and beds were identified using 16S ribosomal RNA (rRNA) gene sequencing analysis. Zero-radius operational taxonomic units (zOTUs) were used for confirming dissemination which indicates bacteria possessing identical sequences within the V3-V4 region of the 16S rRNA gene. Results Ten PIs were analysed in this study. All individuals had zOTUs common to samples from their wound, skin and bed (median: 194, interquartile range [IQR]: 121-320). Furthermore, the bed samples were classified into the same clusters as the wound samples from eight sites.
- Subjects
JAPAN; SKIN microbiology; HOSPITALS; RESEARCH; SEQUENCE analysis; PRESSURE ulcers; CROSS-sectional method; WOUND infections; RNA; BACK; HUMAN microbiota; GENES; DESCRIPTIVE statistics; RESEARCH funding; MICROBIAL contamination; LONG-term health care; BACTERIA; BEDSORE risk factors; DISEASE complications
- Publication
Wound Practice & Research, 2021, Vol 29, Issue 2, p70
- ISSN
1837-6304
- Publication type
Article
- DOI
10.33235/wpr.29.2.70-76