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- Title
Remote conditioning as protective strategy influencing ubiquitinmediated stress response in the rabbit spinal cord after ischemia/reperfusion.
- Authors
Danková, Marianna; Domoráková, Iveta; Fagová, Zuzana; Stebnický, Milan; Mechírová, Eva; Polák, Štefan
- Abstract
The aim of presented study was to investigate the model of non-invasive method of remote conditioning induced by compression of left forelimb with a tourniquet in three cycles of 2 min of ischemia each followed by 2 min of reperfusion and its influence on the rabbit spinal cord ischemia/reperfusion injury via ubiquitin-mediated stress response. Ubiquitin immunoreaction in spinal cord motor neurons as well as detection of neuronal survival in ventral horns of spinal cord were evaluated. Significantly increased (p < 0.001) number of ubiquitin positive neurons was registered in all remote conditioned groups versus both spinal cord ischemia (SC-ischemia) groups. Our results indicate that remote conditioning significantly attenuated degeneration of motor neurons in all conditioned groups versus SC-ischemia groups in each time point. According to our results, we concluded that the remote conditioning induced by transient limb ischemia is relevant stimulus that provides potent neuroprotection in a model of spinal cord ischemia/reperfusion injury.
- Subjects
SPINAL cord; MYOCARDIAL reperfusion; MOTOR neurons; ISCHEMIA; REPERFUSION; REPERFUSION injury; MOTOR neuron diseases
- Publication
General Physiology & Biophysics, 2022, Vol 41, Issue 4, p275
- ISSN
0231-5882
- Publication type
Article
- DOI
10.4149/gpb_2022021