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- Title
Genetic diversity among accessions of an ancient olive variety of Cyprus.
- Authors
Banilas, Georgios; Minas, John; Gregoriou, Costas; Demoliou, Cathrine; Kourti, Anna; Hatzo, Polydefkis
- Abstract
To evaluate germplasm variability and to discriminate between accessions of 'Ladolia', an ancient olive variety of Cyprus, different accessions from a germplasm collection were screened with 11 selected oligonucleotide primers in RAPD-PCRs. A total of 49 polymorphic markers were scored, the combination of which resulted in 70 distinct electrophoretic patterns. Based on either unique or combined patterns, all accessions were identifie. Seven genotype-specific specific markers were detected. One RAPD marker could distriguish accessions according to fruit size. Genetic similarities between accessions, estimated using the Dice similarity coefficient, indicated a high degree of genetic diversity among 'Ladolia' accessions. Genetic relationships were estimated by the unweighted pair-group method with arithmetic averaging (UPGMA) and principal components analysis (PCA). Three main groups of accessions were detected. The first group was generally composed of accessions with small-sized fruits and could be further divided into two subgroups. According to PCA, most accessions with medium- or larged-sized fruits were clustered together. Our results support previous observations suggesting the 'Ladolia' is actually a highly variable mixture of genetically distinct landraces.
- Subjects
GERMPLASM; GENETIC research; GENETIC polymorphisms; OLIVE; GENOTYPE-environment interaction
- Publication
Genome, 2003, Vol 46, Issue 3, p370
- ISSN
0831-2796
- Publication type
Article
- DOI
10.1139/g03-011