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- Title
The melioidosis agent Burkholderia pseudomallei and related opportunistic pathogens detected in faecal matter of wildlife and livestock in northern Australia.
- Authors
HÖGER, A. C. R.; MAYO, M.; PRICE, E. P.; THEOBALD, V.; HARRINGTON, G.; MACHUNTER, B.; CHOY, J. LOW; CURRIE, B. J.; KAESTLI, M.; Höger, A C R
- Abstract
The Darwin region in northern Australia has experienced rapid population growth in recent years, and with it, an increased incidence of melioidosis. Previous studies in Darwin have associated the environmental presence of Burkholderia pseudomallei, the causative agent of melioidosis, with anthropogenic land usage and proximity to animals. In our study, we estimated the occurrence of B. pseudomallei and Burkholderia spp. relatives in faecal matter of wildlife, livestock and domestic animals in the Darwin region. A total of 357 faecal samples were collected and bacteria isolated through culture and direct DNA extraction after enrichment in selective media. Identification of B. pseudomallei, B. ubonensis, and other Burkholderia spp. was carried out using TTS1, Bu550, and recA BUR3–BUR4 quantitative PCR assays, respectively. B. pseudomallei was detected in seven faecal samples from wallabies and a chicken. B. cepacia complex spp. and Pandoraea spp. were cultured from wallaby faecal samples, and B. cenocepacia and B. cepacia were also isolated from livestock animals. Various bacteria isolated in this study represent opportunistic human pathogens, raising the possibility that faecal shedding contributes to the expanding geographical distribution of not just B. pseudomallei but other Burkholderiaceae that can cause human disease.
- Subjects
NORTHERN Australia; AUSTRALIA; MELIOIDOSIS; BURKHOLDERIA pseudomallei; FECES; MICROBIOLOGY; LIVESTOCK diseases; VETERINARY epidemiology; DIAGNOSIS; ANIMAL experimentation; ANIMAL populations; ANIMALS; POLYMERASE chain reaction; TRANSFERASES; GRAM-negative aerobic bacteria
- Publication
Epidemiology & Infection, 2016, Vol 144, Issue 9, p1924
- ISSN
0950-2688
- Publication type
journal article
- DOI
10.1017/S0950268816000285