We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A simplified tumour model established via Epstein-Barr virus-encoded, nasopharyngeal carcinoma-derived oncogene latent membrane protein 1 in immunocompetent mice.
- Authors
Chow, Kai-Ping N.; Wu, C. C.; Chang, H. Y.; Chang, C.; Chang, Y. S.
- Abstract
The expression and immune modulation of Epstein-Barr virus-encoded oncogene latent membrane protein 1 (N-LMP1) is essential in the pathogenesis of nasopharyngeal carcinoma. In previous studies, cell transformation has been induced by the expression of EBV-encoded N-LMP1 in non-tumour BALB/c-3T3 cells and these cells have then been used to form tumours in T-cell-deficient nude mice. However, studies using this model have been limited by the lack of a competent immune system. To facilitate the study of immune components in N-LMP1-driven oncogenesis, we herein developed a simplified N-LMP1-derived tumour model in immunocompetent mice. Cell transformation was induced by the expression of N-LMP1 in BALB/c-3T3 cells, and these transformants were used to induce oncogenesis in BALB/c mice. In contrast to the 100% successful tumour-induction rate in nude mice treated with monodispersed transformed cells, the tumour incidence in BALB/c mice was only 5–36%. However, the transplantation of tumour fragments into BALB/c mice yielded a reproducible tumour-induction rate of >85%, which is acceptable for most of the research needs. This novel model of N-LMP1-directed oncogenesis in an immunocompetent environment may serve as an important platform for the future assessment of N-LMP1-targeted tumour therapies.
- Subjects
EPSTEIN-Barr virus; HERPESVIRUSES; ONCOGENIC DNA viruses; MEMBRANE proteins; LABORATORY mice
- Publication
Laboratory Animals, 2008, Vol 42, Issue 2, p193
- ISSN
0023-6772
- Publication type
Article
- DOI
10.1258/la.2007.006037