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- Title
Studies on the biological effects of ozone: 11. Release of factors from human endothelial cells.
- Authors
Valacchi, Giuseppe; Boccica, Velio
- Abstract
Background: Empirical observations have shown that ozonated autohemotherapy markedly improves the symptoms of chronic limb ischemia (muscular pain at rest, intermittent claudication, etc ) in atherosclerotic patients, but mechanisms of action remain unclear. Aims: Human endothelial cells (HUVECs) are known to release nitrogen monoxide (NO) and we investigated the biological effects of human ozonated serum on HUVECs in culture. Methods: We assessed the relevance of peroxidation, the release of NO as nitrite and of three classical cytokines. Results: The treatment of HUVECs with ozonated serum yields a dose dependent increase of thiobarbituric acid reactive substances (TBARS) and of hydrogen peroxide (H[sub 2]O[sub 2]) and a decrease of protein thiol groups (PTG). Concomitantly, in comparison to either the control or the oxygenated sample, there is a significant and steady increase of nitric oxide (NO) production; this is markedly enhanced by the addition of L-arginine (20μM) and inhibited in the presence of the NO inhibitor, L-NAME (20 m M). The main mediator of ozone action is H[sub 2]O[sub 2] as it has been shown either after its direct measurement or by the addition of 20, 40 and 100 μ M. Moreover, during 24 hours incubation we have investigated the production of endothelin 1 (ET-1), E-selectin and Interleukin 8 (IL-8) and it appears that ozonation enhances IL-8, inhibits E-selectin and hardly modifies ET-1 production. Conclusions: It appears that reinfusion of ozonated blood, by enhancing release of NO, may induce vasodilation in ischemic areas and reduce hypoxia.
- Subjects
OZONE; EFFECT of chemicals on human cell culture; PEROXIDATION; NITROGEN oxides; ANALYTICAL chemistry
- Publication
Mediators of Inflammation, 2000, Vol 9, Issue 6, p271
- ISSN
0962-9351
- Publication type
Article
- DOI
10.1080/09629350020027573