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- Title
Differential Regulation of Epidermal Cell Tumor‐Antigen Presentation by IL‐1α and IL‐1β.
- Authors
Beissert, Stefan; Hosoi, Junichi; Stratigos, Alexander; Brissette, Janice; Grabbe, Stephan; Schwarz, Thomas; Granstein, Richard D.
- Abstract
IL‐1 exists in two forms, termed IL‐1α and IL‐1β, which exert similar effects in a number of biologic models. Recently, there have been reports of some differences in the activities of these two species in some systems. To address this issue with regard to Langerhans cells, Langerhans cell‐enriched preparations of epidermal cells were treated with either IL‐1α or IL‐1β before pulsing with S1509a tumor‐associated antigens and subsequent use for immunization of naïve mice to S1509a. While epidermal cells treated with 100 U IL‐1β per ml were able to induce protective tumor immunity (as indicated by the rejection of a subsequent tumor challenge with viable S1509a tumor cells), epidermal cells treated with 100 U IL‐1α per ml failed to confer protective immunity. At 1000 U per ml, IL‐1β also inhibited the ability of epidermal cells to induce tumor immunity. To investigate the effects of the two IL‐1 forms on elicitation of tumor immunity, naïve mice were immunized against the S1509a tumor by s.c. injection of dead S1509a cells. Epidermal cells enriched for Langerhans cells were treated with either 100 U IL‐1α or IL‐1β per ml before tumor‐associated antigens‐pulsing. Epidermal cells were then washed and injected into a hind footpad of tumor immune mice and 24 h footpad swelling was assessed as a measure of delayed‐type hypersensitivity. Exposure to IL‐1α led to suppressed elicitation of delayed‐type hypersensitivity, whereas IL‐1β treated epidermal cells elicited a normal (100 U per ml) or enhanced (1000 U per ml) level of delayed‐type hypersensitivity. Previous experiments indicated that the suppressive effects of IL‐1α on induction of immunity may be mediated by TNFα. Therefore, the ability of IL‐1α or IL‐1β to induce epidermal cell production of TNFα was assessed. IL‐1α induced epidermal cells to secrete significantly higher amounts of TNFα protein compared with stimulation with IL‐1β. IL‐1α and IL‐1β appear to differentially regulate epidermal cell antigen presenting capability.
- Subjects
INTERLEUKINS; TUMOR antigens; PHYSIOLOGY
- Publication
Journal of Investigative Dermatology, 1998, Vol 111, Issue 4, p609
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1046/j.1523-1747.1998.00291.x