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- Title
Differential Effects of Interferon-γ and Low Molecular Weight BCGF on Growth of Human B Lymphocytes; Interferon-γ Prolongs the Increased c-MYC mRNA Levels After Activation.
- Authors
Lømo, J.; Smeland, E. B.; Stokke, T.; Holte, H.; Funderud, S.; Blomhoff, H. K.
- Abstract
We have characterized the growth-stimulating effect of Interferon-γ (TFN-γ) on various parameters of B cell growth, and compared the effects with those of low molecular weight B cell growth factor (lmw BCGF). We have found that IFN-γ did not affect early changes induced by anti-μ, like initial calcium-flux and rise in mRNA- and protein levels of the proto-oncogene c-myc measured at 3 h. On the other hand, IFN-γ enhanced the effect of anti-μ on parameters measured later in the G1 phase of the cell cycle, such as expression of the transferrin receptor and general transcriptional activity, measured as an increase in 7-aminoactinomycin D binding. In particular, whereas the c-myc levels in anti-μ-treated cells peaked at 3 h and then gradually declined. IFN-γ together with anti-μ maintained the c-myc levels at 24 h at approximately the same levels as seen at 3 h. Overall, lmw BCGF had a more potent effect on the parameters affected by IFN-γ, correlating with stronger enhancement of DNA synthesis. However, in contrast to IFN-γ lmw BCGF did not affect anti-μ-induced c-myc mRNA levels. Thus this study has revealed differences between two B cell growth factors in effects on B cell cycle parameters.
- Subjects
LYMPHOCYTES; B cells; CYTOKINES; MESSENGER RNA; ANTINEOPLASTIC agents; ANTIVIRAL agents
- Publication
Scandinavian Journal of Immunology, 1991, Vol 33, Issue 4, p365
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01783.x