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- Title
Quantitative analysis of a phenanthrene from Eulophia species by TLC-image analysis method.
- Authors
Kanokwan Changwichit; Corine Girard-Thernier; Prapapan Temkitthawon; Nantaka Khorana; Kornkanok Ingkaninan
- Abstract
A phenanthrene constituent in Eulophia macrobulbon (E.C.Parish & Rchb.f.) Hook.f., 1-(4'-hydroxybenzyl)-4,8- dimethoxyphenanthrene-2,7-diol (1) was previously reported as a phosphodiesterase 5 inhibitor. A simple and rapid thin layer chromatographic (TLC)-image analysis method was developed to quantify this compound in plant samples. Chromatographic separation of the phenanthrene was completed on silica gel TLC using dichloromethane-methanol (96:4, v/v) as a mobile phase. Image analysis of the scanned TLC plate was performed by Photoshop CS6 to quantify the amount of phenanthrenes. The area under the spot corresponding to 1 was integrated and used for quantification. The method was validated by the detection limit, calibration curve, repeatability, reproducibility, and recovery. The results showed that our digital imaging method was applicable for quantification of 1 on TLC. The method was convenient, efficient, and moderately accurate for the quantitative analysis of 1 in plant samples.
- Subjects
PHENANTHRENE; EULOPHIA; THIN layer chromatography; IMAGE analysis; PHOSPHODIESTERASE-5 inhibitors; QUANTITATIVE chemical analysis
- Publication
Songklanakarin Journal of Science & Technology, 2018, Vol 40, Issue 6, p1324
- ISSN
0125-3395
- Publication type
Article