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- Title
Immunoregulatory effects of AFP domains on monocyte-derived dendritic cell function.
- Authors
Setiyono, Agus; Budiyati, Akterono D.; Purwantomo, Sigit; Anggelia, Madonna R.; Fanany, Ismail; Wibowo, Gunawan A.; Bachtiar, Indra; Utama, Andi; Susan Tai
- Abstract
Background: Alpha-fetoprotein (AFP) is a tumor-associated glycoprotein that functions in regulation of both ontogenic and oncogenic growth. Recent study showed that AFP can induce apoptosis or impair monocytederived dendritic cell (MDDC) function. However, it is still unclear which AFP domain (D-AFP) plays major role in this function. Results: As expected monocytes cultured in the presence of Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) and Interleukin-4 (IL-4) developed into MDDC. Up-regulation of HLA-DR and CD11c as well as loss of CD14 molecules could be observed. Full length AFP (FL-AFP), domain 2 AFP (D2-AFP) and D3-AFP, but not D1-AFP, significantly inhibited the expression of HLA-DRhigh/CD11chigh and CD80+/CD86high molecules. In contrast, CD83 expression was substantially down-regulated in all samples. Expression of CD40 was significantly suppressed by FL-AFP but not by any D-AFPs. Finally, both FL-AFP and D-AFP impaired the MDDC ability to secrete IL-12 (p70). Conclusions: D2- and D3- but not D1-AFP extensively suppresses the MDDC function. All the recombinant AFP proteins impaired the ability of MDDC to secrete IL-12.
- Subjects
ALPHA fetoproteins; DENDRITIC cells; APOPTOSIS; GRANULOCYTE-macrophage colony-stimulating factor; MONOCYTES
- Publication
BMC Immunology, 2011, Vol 12, Issue 1, p1
- ISSN
1471-2172
- Publication type
Article
- DOI
10.1186/1471-2172-12-4