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- Title
Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response.
- Authors
Crabbé, Laure; Thomas, Aubin; Pantesco, Véronique; De Vos, John; Pasero, Philippe; Lengronne, Armelle
- Abstract
Maintenance of genome integrity relies on surveillance mechanisms that detect and signal arrested replication forks. Although evidence from budding yeast indicates that the DNA replication checkpoint (DRC) is primarily activated by single-stranded DNA (ssDNA), studies in higher eukaryotes have implicated primer ends in this process. To identify factors that signal primed ssDNA in Saccharomyces cerevisiae, we have screened a collection of checkpoint mutants for their ability to activate the DRC, using the repression of late origins as readout for checkpoint activity. This quantitative analysis reveals that neither RFCRad24 and the 9-1-1 clamp nor the alternative clamp loader RFCElg1 is required to signal paused forks. In contrast, we found that RFCCtf18 is essential for the Mrc1-dependent activation of Rad53 and for the maintenance of paused forks. These data identify RFCCtf18 as a key DRC mediator, potentially bridging Mrc1 and primed ssDNA to signal paused forks.
- Subjects
DNA replication; YEAST; SACCHAROMYCES cerevisiae; QUANTITATIVE research; DNA damage; EUKARYOTIC cells
- Publication
Nature Structural & Molecular Biology, 2010, Vol 17, Issue 11, p1391
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.1932