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- Title
High Throughput Ion-Channel Pharmacology: Planar-Array-Based Voltage Clamp.
- Authors
Kiss, Laszlo; Bennett, Paul B.; Uebele, Victor N.; Koblan, Kenneth S.; Kane, Stefanie A.; Neagle, Brad; Schroeder, Kirk
- Abstract
Technological advances often drive major breakthroughs in biology. Examples include PCR, automated DNA sequencing, confocal/single photon microscopy, AFM, and voltage/patch-clamp methods. The patch-clamp method, first described nearly 30 years ago, was a major technical achievement that permitted voltage-clamp analysis (membrane potential control) of ion channels in most cells and revealed a role for channels in unimagined areas. Because of the high information content, voltage clamp is the best way to study ion-channel function; however, throughput is too low for drug screening. Here we describe a novel breakthrough planar-array-based HT patch-clamp technology developed by Essen Instruments capable of voltage-clamping thousands of cells per day. This technology provides greater than two orders of magnitude increase in throughput compared with the traditional voltage-clamp techniques. We have applied this method to study the hERG K+ channel and to determine the pharmacological profile of QT prolonging drugs.
- Publication
Assay & Drug Development Technologies, 2003, Vol 1, p127
- ISSN
1540-658X
- Publication type
Article
- DOI
10.1089/154065803321537845