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- Title
miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells.
- Authors
Shi, Yu; Mao, Xudong; Cai, Mingcheng; Hu, Shenqiang; Lai, Xiulan; Chen, Shiyi; Jia, Xianbo; Wang, Jie; Lai, Songjia
- Abstract
Skeletal muscle satellite cells (SMSCs), also known as a multipotential stem cell population, play a crucial role during muscle growth and regeneration. In recent years, numerous miRNAs have been associated with the proliferation and differentiation of SMSCs in a number of mammalian species; however, the regulatory mechanisms of miR-194-5p in rabbit SMSCs still remain scarce. In this study, miR-194-5p was first observed to be highly expressed in the rabbit leg muscle. Furthermore, both the mimics and inhibitor of miR-194-5p were used to explore its role in the proliferation and differentiation of rabbit SMSCs cultured in vitro. Results from both EdU and CCK8 assays showed that miR-194-5p inhibited the proliferation of SMSCs. Meanwhile, Mef2c was identified as a target gene of miR-194-5p based on the dual-luciferase reporter assay results. In addition, upregulation of miR-194-5p decreased the expression levels of Mef2c and MyoG during rabbit SMSCs differentiation on Days 3 and 7 of in vitro culture. Taken together, these data demonstrated that miR-194-5p negatively regulates the proliferation and differentiation of rabbit SMSCs by targeting Mef2c.
- Subjects
SATELLITE cells; MUSCLE cells; SKELETAL muscle; RABBITS; CELL populations
- Publication
Molecular & Cellular Biochemistry, 2021, Vol 476, Issue 1, p425
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-020-03918-0