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- Title
Heterogeneous expression and emulsifying activity of class I hydrophobin from Pholiota nameko.
- Authors
Kudo, Takunobu; Sato, Yuki; Tasaki, Yuji; Hara, Takashi; Joh, Toshio
- Abstract
Hydrophobins secreted by filamentous fungi self-assemble into an amphipathic film at hydrophilic/hydrophobic interfaces. This unique property suggests that the hydrophobins have a high potential for industrial applications. However, the assemblages of class I hydrophobins are highly insoluble, making such commercial applications difficult. To enhance the solubility of class I hydrophobins, we have attempted to express class I hydrophobin PNH1 from Pholiota nameko fused with glutathione S-transferase (GST) in Escherichia coli. The GST-PNH1 was effectively isolated from the soluble fraction of transformed E. coli, and subsequent analysis revealed that the purified GST-PNH1 had almost the same emulsifying activity as PNH1.
- Subjects
PHOLIOTA; HYDROPHOBINS; FILAMENTOUS fungi; BASIDIOMYCETES; EMULSIONS; GLUTATHIONE transferase; ESCHERICHIA coli; SOLUBILITY
- Publication
Mycoscience (Springer Nature), 2011, Vol 52, Issue 4, p283
- ISSN
1340-3540
- Publication type
Article
- DOI
10.47371/s10267-010-0097-9