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- Title
Targeted imaging of cancer by fluorocoxib C, a near-infrared cyclooxygenase-2 probe.
- Authors
Uddin, Md. Jashim; Crews, Brenda C.; Ghebreselasie, Kebreab; Daniel, Cristina K.; Kingsley, Philip J.; Shu Xu; Marnett, Lawrence J.
- Abstract
Cyclooxygenase-2 (COX-2) is a promising target for the imaging of cancer in a range of diagnostic and therapeutic settings. We report a near-infrared COX-2-targeted probe, fluorocoxib C (FC), for visualization of solid tumors by optical imaging. FC exhibits selective and potent COX-2 inhibition in both purified protein and human cancer cell lines. In vivo optical imaging shows selective accumulation of FC in COX-2-overexpressing human tumor xenografts [1483 head and neck squamous cell carcinoma (HNSCC)] implanted in nude mice, while minimal uptake is detectable in COX-2-negative tumor xenografts (HCT116) or 1483 HNSCC xenografts preblocked with the COX-2-selective inhibitor celecoxib. Time course imaging studies conducted from 3 h to 7-day post-FC injection revealed a marked reduction in nonspecific fluorescent signals with retention of fluorescence in 1483 HNSCC tumors. Thus, use of FC in a delayed imaging protocol offers an approach to improve imaging signal-to-noise that should improve cancer detection in multiple preclinical and clinical settings.
- Subjects
CYCLOOXYGENASE 2; CANCER research; DIAGNOSTIC imaging; CYCLOOXYGENASES; OXIDOREDUCTASES
- Publication
Journal of Biomedical Optics, 2015, Vol 20, Issue 5, p1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.20.5.050502