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- Title
Increased production of γ-lactones from hydroxy fatty acids by whole Waltomyces lipofer cells induced with oleic acid.
- Authors
An, Jung-Ung; Oh, Deok-Kun
- Abstract
Among several fatty acids tested, oleic acid was selected as the most efficient inducer for the production of 4-hydroxydodecanoic acid, a metabolite of β-oxidation, by Waltomyces lipofer. Cells were induced by incubation for 12 h in a medium containing 10 g l yeast extract, 10 g l peptone, 5 g l oleic acid, 1 g l glucose, and 0.05 % ( w/ v) Tween 80. The optimal reaction conditions for the production of γ-lactones by induced cells were pH 6.5, 35 °C, 200 rpm, 0.71 M Tris, 60 g l hydroxy fatty acid, and 20 g l cells. Non-induced cells produced 38 g l γ-dodecalactone from 60 g l 10-hydroxystearic acid after 30 h, with a conversion yield of 63 % ( w/ w) and a productivity of 1.3 g l h under the optimized conditions, whereas induced cells produced 51 g l γ-dodecalactone from 60 g l 10-hydroxystearic acid after 30 h, with a conversion yield of 85 % ( w/ w) and a productivity of 1.7 g l h. The conversion yield and productivity of induced cells were 22 % and 1.3-fold higher, respectively, than those of non-induced cells. Induced cells also produced 28 g l γ-decalactone and 12 g l γ-butyrolactone from 60 g l 12-hydroxystearic acid and 60 g l 10-hydroxydecanoic acid, respectively, after 30 h. The concentration, conversion yield, and productivity of γ-dodecalactone and γ-decalactone are the highest reported thus far. This is the first study on the biotechnological production of γ-butyrolactone.
- Subjects
LACTONES; HYDROXY acids; FATTY acids; OLEIC acid; BUTYROLACTONES; STEARIC acid; BIOTECHNOLOGICAL microorganisms
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 18, p8265
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-5089-1