We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of PCR-based chloroplast DNA markers that characterize domesticated cowpea ( Vigna unguiculata ssp. unguiculata var. unguiculata) and highlight its crop-weed complex.
- Authors
Feleke, Y.; Pasquet, R. S.; Gepts, P.
- Abstract
In 1992, Vaillancourt and Weeden discovered a very important mutation for studying cowpea evolution and domestication. A loss of a BamHI restriction site in chloroplast DNA characterized all domesticated accessions and a few wild ( Vigna unguiculata ssp. unguiculata var. spontanea) accessions. In order to screen a larger number of accessions, primers were designed to check this mutation using PCR RFLP or direct PCR methods. Using these new primers, 54 domesticated cowpea accessions and 130 accessions from the wild progenitor were screened. The absence of haplotype 0 was confirmed within domesticated accessions, including primitive landraces from cultivar-groups Biflora and Textilis, suggesting that this mutation occurred prior to domestication. However, 40 var. spontanea accessions distributed from Senegal to Tanzania and South Africa showed haplotype 1. Whereas this marker could not be used to identify a precise center of origin, it did highlight the widely distributed cowpea crop-weed complex. Its very high frequency in West Africa could be interpreted as a result of either genetic swamping of the wild/weedy gene pool by the domesticated cowpea gene pool or as the result of domestication by ethnic groups focusing primarily on cowpea as fodder.
- Subjects
COWPEA; GENETIC markers; POLYMERASE chain reaction; PLANT classification; PLANT evolution
- Publication
Plant Systematics & Evolution, 2006, Vol 262, Issue 1/2, p75
- ISSN
0378-2697
- Publication type
Article
- DOI
10.1007/s00606-006-0475-0