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- Title
Bone Cell Autophagy Is Regulated by Environmental Factors.
- Authors
Zahm, Adam M.; Bohensky, Jolene; Adams, Christopher S.; Shapiro, Irving M.; Srinivas, Vickram
- Abstract
The goal of this investigation was to ascertain whether bone cells undergo autophagy and to determine if this process is regulated by environmental factors. We showed that osteocytes in both murine and human cortical bone display a punctuate distribution of microtubule-associated protein light chain 3, indicative of autophagy. In addition, we noted a basal level of autophagy in preosteocyte-like murine long bone-derived osteocytic (MLO)-A5 cells. Autophagy was upregulated following nutrient deprivation and hypoxic culture, stress conditions that osteocytes encounter in vivo. Furthermore, in response to calcium stress, the transcription factor hypoxia inducible factor 1 regulated MLO-A5 autophagy. Finally, we showed that the more differentiated MLO-Y4 osteocyte-like cells exhibited a significant basal autophagic flux. Based on these findings, we suggest that raising the level of autophagic flux is a mechanism by which differentiated bone cells survive in a stressful environment. Copyright © 2011 S. Karger AG, Basel
- Subjects
BONE cells; HYPOXEMIA; AUTOPHAGY; TUBULINS; OSTEOCYTES; LABORATORY rats
- Publication
Cells Tissues Organs, 2011, Vol 194, Issue 2-4, p274
- ISSN
1422-6405
- Publication type
Article
- DOI
10.1159/000324647