We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of surfact in interactome using Escherichia coli proteome chip.
- Authors
Hung, L. Y.; Tseng, C. Y.; Yu, Y. H.; Cheng, Y. H.; Chen, C. S.; Horng, Y. B.; Lin, T. Y.; Peng, T. C.; Hsiao, S. H.
- Abstract
Escherichia coli is a common environmental pathogen that often induces mastitis causes economic losses to dairy farmers. Surfactin is composed of hydrophilic and hydrophobic parts, which can effectively decompose bacterial membranes. Some studies show surfactin can inhibit pathogen virulence. However, the interactome between surfactin and E. coli is still unknown. First, this study explored the sensitivity of E. coli with surfactin, and surfactin could inhibit E. coli growth (P< 0.05) within 2 hours of co-culture. Afterwards, we used D. coli proteome chip to exploreinteraction with surfactin. We found 119 bacterial proteins specifically bind with surfactin (hits protein). The hits and E. coli were associated in organic substance process with metabolism and bacterial antigen with capsule by Gene Ontology, alsothe hits in "Antibiotic biosynthesis" and "Secondary metabolites biosynthesis" were enriched (P < 0.05) by KEGG assay. In addition, the STRING shows their interrelationships such as targeting regulation among hits protein, and they have significant repetitive variable sequences (P < 0.0001) by Motif. Collectively, this study identified the surfactin interactome, which is beneficial to explore the antibacterial of E. coli and analyze the molecular mechanism of E. coli mastitis.
- Subjects
ESCHERICHIA coli; PROTEOMICS; BACTERIAL cell walls; SURFACTIN; GENE ontology; BIOSYNTHESIS
- Publication
Journal of the Chinese Society of Animal Science, 2021, Vol 50, p143
- ISSN
0253-9187
- Publication type
Article