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- Title
Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells.
- Authors
Lee, Da-Yeon; Chun, Yoon-Seok; Kim, Jong-Kyu; Lee, Jeong-Ok; Lee, Young-Joon; Ku, Sae-Kwang; Shim, Soon-Mi; Fulle, Stefania
- Abstract
The purpose of the current study was to investigate antioxidant and anti-inflammatory effects of spray dry powder containing 40% curcumin (CM-SD) in C2C12 myoblast cells. CM-SD increased DPPH radical scavenging activity in a dose-dependent manner, and up to 30 μg/mL of CM-SD did not express cytotoxicity in C2C12 cells. Exposure to hydrogen peroxide (H2O2) drastically decreased the viability of C2C12 cells, but pre-treatment of CM-SD significantly increased the cell viability (p < 0.01). CM-SD significantly transactivated the nuclear factor erythroid-2-related factor 2 (Nrf2)-dependent luciferase activity in a dose-dependent manner and enhanced the levels of heme oxygenase (HO)-1, glutamate cysteine ligase catalytic subunit (GCLC), and NAD(P)H-dependent quinone oxidoreductase (NQO)-1. CM-SD also significantly reduced reactive oxygen species (ROS) production and lipid peroxidation and restored glutathione (GSH) depletion in H2O2-treated C2C12 cells. Moreover, CM-SD significantly reduced lipopolysaccharides (LPS)-mediated interleukin (IL)-6 production in the conditioned medium. Results from the current study suggest that CM-SD could be a useful candidate against oxidative stress and inflammation-related muscle disorders.
- Subjects
OXIDATIVE stress; CURCUMIN; HEME oxygenase; LUCIFERASES; SPRAY drying; REACTIVE oxygen species; QUINONE; MYOBLASTS
- Publication
Antioxidants, 2021, Vol 10, Issue 3, p476
- ISSN
2076-3921
- Publication type
Article
- DOI
10.3390/antiox10030476