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- Title
Antimicrobial and antivirulence saponins of Mimusops laurifolia leaves.
- Authors
Mostafa, Hayam M A; Taha, Mohamed; El-Gendy, Ahmed O; Khairalla, Ahmed S; abd El fattah, Medhat; Raslan, Mai
- Abstract
Mimusops laurifolia is a native species restricted to the Red Sea mountains and Gulf of Aden. Its leaves contain saponins with wide range of biological activities. The presented research aimed to prepare saponins-rich extract from n -butanol fraction of M. laurifolia leaves and screen it for promising antimicrobial activities. Minimum inhibitory concentration (MIC) of the prepared saponins against Candida albicans , and their potential anti-pathogenic and antivirulence effects were determined. Different concentrations of the saponins-rich extract were investigated for their antimicrobial potential, particularly against C. albicans , using the agar well diffusion method. To assess the potential antivirulence and antipathogenic effects, we carried out molecular docking of the bioactive saponins against four key enzymes in C. albicans , which are involved in virulence and/or pathogenicity. Different concentrations of the investigated mixture showed notable antifungal activity against C. albicans with an MIC value of 6.4 μg ml−1. Docking analysis of the investigated saponins showed their affinity toward the docked enzymes, particularly saponin 1 with secreted aspartic proteinase 3 and saponin 6 with secreted aspartic proteinase 5. Thereafter, the stability of these two protein-ligand interactions was investigated using molecular dynamics (MD) simulation. The molecular interactions between saponins and the enzymes' active sites were analyzed and discussed.
- Subjects
GULF of Aden; SAPONINS; ASPARTIC proteinases; BINDING sites; BUTANOL; PROTEIN-ligand interactions; MOLECULAR docking; ANTIFUNGAL agents; CANDIDA albicans
- Publication
Letters in Applied Microbiology, 2023, Vol 76, Issue 7, p1
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1093/lambio/ovad071