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- Title
Unraveling Low-Level Gamma Radiation–Responsive Changes in Expression of Early and Late Genes in Leaves of Rice Seedlings at litate Village, Fukushima.
- Authors
Hayashi, Gohei; Shibato, Junko; Imanaka, Tetsuji; Cho, Kyoungwon; Kubo, Akihiro; Kikuchi, Shoshi; Satoh, Kouji; Kimura, Shinzo; Ozawa, Shoji; Fukutani, Satoshi; Endo, Satoru; Ichikawa, Katsuki; Agrawal, Ganesh Kumar; Shioda, Seiji; Fukumoto, Manabu; Rakwal, Randeep
- Abstract
In the summer of 2012, 1 year after the nuclear accident in March 2011 at the Fukushima Daiichi nuclear power plant, we examined the effects of gamma radiation on rice at a highly contaminated field of Iitate village in Fukushima, Japan. We investigated the morphological and molecular changes on healthy rice seedlings exposed to continuous low-dose gamma radiation up to 4 µSv h−1, about 80 times higher than natural background level. After exposure to gamma rays, expression profiles of selected genes involved in DNA replication/repair, oxidative stress, photosynthesis, and defense/stress functions were examined by RT-PCR, which revealed their differential expression in leaves in a time-dependent manner over 3 days (6, 12, 24, 48, and 72h). For example, OsPCNA mRNA rapidly increased at 6, 12, and 24h, suggesting that rice cells responded to radiation stress by activating a gene involved in DNA repair mechanisms. At 72h, genes related to the phenylpropanoid pathway (OsPAL2) and cell death (OsPR1oa) were strongly induced, indicating activation of defense/stress responses. We next profiled the transcriptome using a customized rice whole-genome 4×44K DNA microarray at early (6h) and late (72h) time periods. Low-level gamma radiation differentially regulated rice leaf gene expression (induced 4481 and suppressed 3740 at 6h and induced 2291 and suppressed 1474 genes at 72h) by at least 2-fold. Using the highly upregulated and downregulated gene list, MapMan bioinformatics tool generated diagrams of early and late pathways operating in cells responding to gamma ray exposure. An inventory of a large number of gamma radiation–responsive genes provides new information on novel regulatory processes in rice.
- Subjects
GAMMA rays; RICE; DNA repair; RADIATION &; the environment; GENOMICS
- Publication
Journal of Heredity, 2014, Vol 105, Issue 5, p723
- ISSN
0022-1503
- Publication type
Article
- DOI
10.1093/jhered/esu025