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- Title
维生素C对创伤性脑损伤后神经细胞焦亡及NLRP3通路的影响.
- Authors
陈燕伟; 武秀权; 王利; 李侠; 林伟; 蒋晓帆; 张磊
- Abstract
Objective: To observed the effect and molecular mechanism of vitamin C(Vit C) pretreatment against nerve cell pyroptosis in traumatic brain injury (TBI). Methods: The HT22 cell lines were randomly divided into the sham group (sham group), DMEM control group (Con group) and the Vit C pretreatment group (Vit C group); Con group and Vit C group pretreatment in 72 h, then the TBI model was induced by scratch injury in the groups for 12 h and then sampling. The expression of NLRP3 was detected by immunofluorescence staining and Western-blot; The standard ELISA kit was used to detect the expression of IL-1β and IL-18. Results: Compared with the Sham group, the positive rate of NLRP3 staining in surviving neurons was high in the Con group and the Vit C groups; Compared with Con group, low and high dose Vit C can obviously reduce the expression level of NLRP3 protein, IL-1β and IL-18(P<0.05), and the difference among the low dose and the high dose groups was statistically significant (P<0.05). Conclusions: Different doses of Vit C effectively reduce nerve cell pyroptosis after TBI, possibly by alleviating the expression level of NLRP3, reducing the release of IL-1β and IL-18, and play important neuroprotective effects.
- Subjects
NLRP3 protein; BRAIN injuries; NEURONS; VITAMIN C; CELL lines
- Publication
Progress in Modern Biomedicine, 2021, Vol 21, Issue 24, p4632
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2021.24.006