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- Title
Cloning and Analysis of Anti-stress Transcription Factor Gene HhDREB2 from Halimodendron halodendron. Voss.
- Authors
LEI Zhi; YIN Cui-cui; LI Yong-liang; SUN Zhan-min; WU Yan-min
- Abstract
In this study, a full-length cDNA sequence of DREB transcription factor gene, HhDREB2 (GenBank No.: EU872018), was isolated from Halimodendron halodendron. Voss by homology-based cloning and RACE (rapid amplification of cDNA end) technology. Sequence analysis showed that the cDNA sequence was 873 bp without introns and had a maximum open reading frame from 108 to 626 bp, encoding 172 amino acid residues. Molecular weight of the gene encoding protein was 19.702 kDa, isoelectric point was 8.92. Phylogenetic tree and homologous sequence alignment analysis indicated that this protein contained a typical AP2/ERF conservative domain, and was most closely related to soybean GmDREB2, belonging to the A5 subgroups. Yeast one-hybrid experiment demonstrated that HhDREB2 gene encoded a transcription activator. In addition, subcellular localization analysis of a construct with the transient expression vector of the HhDREB2 gene showed that the HhDREB2 protein was localized in the nucleus. The expression level of HhDREB2 gene was greatly induced by high salt, drought and low temperature through semi-quantitative RT-PCR, and had no significant change with GA3, NAA, ABA and 6BA treatments.
- Subjects
ANTISENSE DNA; TRANSCRIPTION factors; AMINO acids; PLANT cloning; PLANT genes
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2014, Vol 16, Issue 4, p71
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2014.083