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- Title
CDK4 has the ability to regulate Aurora B and Cenpp expression in mouse keratinocytes.
- Authors
Lee, Sung Hyun; Rodriguez, Liliana R.L.; Majumdar, Rima; De Marval, Paula L. Miliani; Rodriguez-Puebla, Marcelo L.
- Abstract
Cyclin-dependent kinase 4 (CDK4) is a critical molecule that regulates key aspects of cell proliferation through phosphorylation of the retinoblastoma (Rb) family of proteins. In the last few years, it has been suggested that CDK4 plays alternative roles in cell proliferation and tumorigenesis. The main aim of the present study was to define a novel CDK4 function as a transcriptional regulator of genes involved in chromosome segregation, contributing to the G2/M phase transition. Herein, chromatin-immunoprecipitation reverse transcription-quantitative PCR assays were performed to demonstrate that CDK4 could occupy the promoter region of genes associated with chromosomal segregation, such as Aurora-B (Aurkb) and Centromere Protein P (CENP-P). Moreover, gain- and loss-of-function experiments showed that CDK4 participated in the transcriptional regulation of Aurkb and CENP-P. The finding that Aurkb may have a crucial role in chromosome bi-orientation and the spindle assembly checkpoint, and that CENP-P could be required for proper kinetochore function suggests that dysregulation of CDK4 expression induces chromosomal instability and, in some cases, cancer development.
- Subjects
CYCLIN-dependent kinases; AURORA kinases; KERATINOCYTES; REGULATOR genes; CHROMOSOME segregation
- Publication
Oncology Letters, 2021, Vol 22, Issue 4, pN.PAG
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2021.12993