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- Title
Cytokine-induced prostaglandin E<sub>2</sub> production and cyclooxygenase-2 expression in dental pulp cells: downstream calcium signalling via activation of prostaglandin EP receptor.
- Authors
Chang, M.‐C.; Chen, Y.‐J.; Tai, T.‐F.; Tai, M.‐R.; Li, M.‐Y.; Tsai, Y.‐L.; Lan, W.‐H.; Wang, Y.‐L.; Jeng, J.‐H.
- Abstract
Aim To determine whether (i) proinflammatory cytokines stimulate prostaglandin E2 (PGE2) production and cyclooxygenase (COX) gene expression in dental pulp cells, and (ii) pulp cells that express different prostaglandin E2 receptor (EP) isoforms and their activation by PGE2 leads to downstream Ca2+ signalling. Methodology Cultured human dental pulp cells were exposed to interleukin (IL)-1 β and tumour necrotic factor-alpha (TNF- α). The expression of COX-1 and COX-2 was measured with reverse transcriptase-polymerase chain reaction (RT-PCR). The production of PGE2 was measured using an enzyme-linked immunosorbent assay. Expression of prostaglandin EP receptor isoforms was studied by RT-PCR, whereas fura-2 fluorescence was used to measure calcium mobilization. The Kruskal–Wallis test and Wilcoxon sum rank test with Bonferroni correction were used for statistical analysis. Results Interleukin-1 β and TNF- α stimulate PGE2 production of human dental pulp cells ( P < 0.05). IL-1 β stimulated the COX-2 but not COX-1 mRNA expression. Pulp cells express mainly EP2, EP3 and EP1 receptors as analysed by RT-PCR. PGE2 (0.25–2 μmol L−1) stimulated the Ca2+ mobilization as indicated by increase in fura-2 fluorescence. Conclusions Interleukin-1 β and TNF- α may stimulate PGE2 production in dental pulp cells. Activation of prostaglandin EP receptors in dental pulp cells by PGE2 may induce Ca2+ signalling to regulate cellular biological activity during inflammation.
- Subjects
DENTAL pulp; CYTOKINES; GENE expression; MESSENGER RNA; DENTIN; INTERLEUKINS
- Publication
International Endodontic Journal, 2006, Vol 39, Issue 10, p819
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/j.1365-2591.2006.01156.x