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- Title
Detection of minor constituents in TongMai Granules using NP×RP off-line two-dimensional liquid chromatography coupled with orbitrap mass spectrometry.
- Authors
Lulu Xu; Zhanpeng Shang; Yue Chai; Yang Zhang; Rong Yu; Min Ye; Xue Qiao
- Abstract
TongMai Granules is a Chinese medicine patent drug to treat cardiovascular diseases. It is prepared from the water extract of Dan-shen, Chuan-xiong and Ge-gen. The main compounds of TongMai Granules are isoflavone glycosides, isoflavones, and phenolic acids. However, it is difficult to detect low-polar compounds such as tanshinones and phthalides though they may also contribute to the therapeutic effect. In this work, an off-line two-dimensional chromatography method was developed to enrich the minor constituents in TongMai Granules. The extract was subjected to an SEP-PAK silica SPE column and eluted with petroleum ether, ethyl acetate-methanol (6:1, v/v), and methanol, successively. The three fractions were then analyzed using ultra-high performance liquid chromatography coupled with orbitrap mass spectrometry (UHPLC/orbitrap-MS). The isoflavones from Ge-gen, phenolic acids from Dan-shen and phthalides from Chuan-xiong were distributed in different fractions. A total of 131 compounds, including 59 from Ge-gen, 26 from Dan-shen, 43 from Chuan-xiong, and 3 common ones from Dan-shen and Chuan-xiong were tentatively identified. Among them, 26 compounds were identified by comparing with reference standards. This study set a good example for comprehensive analysis of the constituents in traditional Chinese medicine formula.
- Subjects
LIQUID chromatography; MASS spectrometry; TREATMENT effectiveness; CHINESE medicine; DRUG patents; GRANULATION; ETHYL acetate; PHENOLIC acids
- Publication
Journal of Chinese Pharmaceutical Sciences, 2020, Vol 29, Issue 11, p793
- ISSN
1003-1057
- Publication type
Article
- DOI
10.5246/jcps.2020.11.072