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- Title
Screening and Identification of Tobacco Mutants Resistant to Potato Virus Y (PVY).
- Authors
Xiuqing WAN; Guoshun WU; Chan QIAO; Ruo LI; Lei HUANG
- Abstract
[Obbective] This study aimed to screen and identify tobacco mutants resistant to potato virus Y (PVY), thus laying the foundation for obtaining PVY resistance genes. [Method] At seedling stage, tobacco mutant materials were inoculated with PVY virus and preliminarily screened by naked-eye observation. Enzyme- linked immunosorbent assay (ELISA), real-time fluorescence quantitative PCR and test strip assay were performed to further identify the pre-screened PVYresistant seedlings. [Result] In 2011, two highly PVY-resistant tobacco mutants (MZE2-15 ad MZE2-16) and three PVY-tolerant mutants (MZE2-70, MZE2- 207 and MZE2-228) were obtained, which were further screened and identified in 2012. According to the results, tobacco mutant materials MZE2-07 and MZE2- 428 were susceptible to PVY; mutant materials MZE2-16 and MZE2-15 were resistant to PVY. [ Conclusion] This study provide theoretical bais for the control of tobacco PVY disease.
- Subjects
POTATO virus Y; TOBACCO disease &; pest resistance; POTYVIRUSES; PLANT genes; PLANT genetics; GENETIC research
- Publication
Agricultural Biotechnology (2164-4993), 2015, Vol 4, Issue 5, p1
- ISSN
2164-4993
- Publication type
Article