We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
NMR, Novel Pharmacological and In Silico Docking Studies of Oxyacanthine and Tetrandrine: Bisbenzylisoquinoline Alkaloids Isolated from Berberis glaucocarpa Roots.
- Authors
Alamzeb, Muhammad; Omer, Muhammad; Ur-Rashid, Mamoon; Raza, Muslim; Ali, Saqib; Khan, Behramand; Ullah, Asad
- Abstract
Urease enzyme is responsible for gastric cancer, peptic ulcer, hepatic coma, and urinary stones in millions of people across the world. So, there is a strong need to develop new and safe antiurease drugs, particularly from natural sources. In search for new and effective drugs from natural sources bioassay-guided fractionation and isolation of <italic>Berberis glaucocarpa</italic> Stapf roots bark resulted in the isolation and characterization, on the basis of 1D and 2D NMR data, of two bisbenzylisoquinoline alkaloids, oxyacanthine (<bold>1</bold>) and tetrandrine (<bold>2</bold>), followed by urease inhibition studies. Crude extract, all the subfractions and the isolated compounds <bold>1</bold> and <bold>2</bold> displayed excellent urease enzyme inhibition properties <italic>in vitro</italic>. The antiurease nature and possible mode of action for compounds <bold>1</bold> and <bold>2</bold> were verified and explained through their molecular docking studies against jack-bean urease enzyme. Half-maximum inhibitory concentration (IC50) was calculated for compounds <bold>1</bold> and <bold>2</bold>. The IC50 value was found to be 6.35 and 5.51 <italic>µ</italic>g/mL for compounds <bold>1</bold> and <bold>2</bold>, respectively. Both compounds <bold>1</bold> and <bold>2</bold> have minimal cytotoxicity against THP-1 monocytic cells.
- Subjects
TETRANDRINE; BISBENZYLISOQUINOLINE alkaloids; BARBERRIES; PLANT extracts; UREASE; ENZYME inhibitors
- Publication
Journal of Analytical Methods in Chemistry, 2018, p1
- ISSN
2090-8865
- Publication type
Article
- DOI
10.1155/2018/7692913