We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Highly Efficient Sex Chromosome Interchanges Produced By I-Crel Expression in Drosophila.
- Authors
Maggert, Keith A.; Golic, Kent G.
- Abstract
The homing endonuclease I-CreI recognizes a site in the gene encoding the 23S rRNA of Chlamydomonas reinhardtii. A very similar sequence is present in the 28S rRNA genes that are located on the X and Y chromosomes of Drosophila melanogaster. In this work we show that I-CreI expression in Drosophila is capable of causing induced DNA damage and eliciting cell cycle arrest. Expression also caused recombination between the X and Y chromosomes in the heterochromatic regions where the rDNA is located, presumably as a result of a high frequency of double-strand breaks in these regions. Approximately 20% of the offspring of males expressing I-CreI showed exceptional inheritance of X- and Y-linked markers, consistent with chromosome exchange at rDNA loci. Cytogenetic analysis confirmed the structures of many of these products. Exchange between the X and Y chromosomes can be induced in males and females to produce derivative-altered V chromosomes, attached-XY, and attached-X chromosomes. This method has advantages over the traditional use of X rays for generating X-Y interchanges because it is very frequent and it generates predictable products.
- Subjects
DROSOPHILA; SEX chromosomes; CHLAMYDOMONAS reinhardtii; RNA; X chromosome; Y chromosome
- Publication
Genetics, 2005, Vol 171, Issue 3, p1103
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.104.040071