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- Title
Analysis of Ras-Induced Overproliferation in Drosophila Hemocytes.
- Authors
Asha, H.; Nagy, Istvan; Kovacs, Gabor; Stetson, Daniel; Ando, Istvan; Dearolf, Charles R.
- Abstract
We use the Drosophila melanogaster larval hematopoietic system as an in vivo model for the genetic and functional genomic analysis of oncogenic cell overproliferation. Ras regulates cell proliferation and differentiation in multicellular eukaryotes. To further elucidate the role of activated Ras in cell overproliferation, we generated a collagen promoter-Gal4 strain to overexpress Ras[sup V12] in Drosophila hemocytes. Activated Ras causes a dramatic increase in the number of circulating larval hemocytes (blood cells), which is caused by cellular overproliferation. This phenotype is mediated by the Raf/MAPK pathway. The mutant hemocytes retain the ability to phagocytose bacteria as well as to differentiate into lamellocytes. Microarray analysis of hemocytes overexpressing Ras[sup V12] vs. Ras[sup +] identified 279 transcripts that are differentially expressed threefold or more in hemocytes expressing activated Ras. This work demonstrates that it will be feasible to combine genetic and functional genomic approaches in the Drosophila hematopoietic system to systematically identify oncogene-specific downstream targets.
- Subjects
DROSOPHILA; RAS oncogenes; CELL proliferation
- Publication
Genetics, 2003, Vol 163, Issue 1, p203
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1093/genetics/163.1.203