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- Title
Antibacterial Activity and Mode of Action of Lactoquinomycin A from Streptomyces bacillaris.
- Authors
Chung, Beomkoo; Kwon, Oh-Seok; Shin, Jongheon; Oh, Ki-Bong
- Abstract
This study aims to isolate and identify the structure of antibacterial compounds having potent activity on methicillin-resistant Staphylococcus aureus (MRSA) from marine actinomycetes, and also to identify their mode of action. Lactoquinomycin A (LQM-A) (compound 1) and its derivatives (2–4) were isolated from marine-derived Streptomyces bacillaris strain MBTC38, and their structures were determined using extensive spectroscopic methods. These compounds showed potent antibacterial activities against Gram-positive bacteria, with MIC values of 0.06–4 μg/mL. However, the tested compounds exhibited weak inhibitory activity against Gram-negative bacteria, although they were effective against Salmonella enterica (MIC = 0.03–1 μg/mL). LQM-A exhibited the most significant inhibitory activity against methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 0.25–0.5 μg/mL), with a low incidence of resistance. An in vivo dual-reporter assay designed to distinguish between compounds that inhibit translation and those that induce DNA damage was employed to assess the mode of action of LQM-A. LQM-A-induced DNA damage and did not inhibit protein synthesis. The gel mobility shift assay showed that LQM-A switched plasmid DNA from the supercoiled to relaxed form in a time- and concentration-dependent manner. These data suggest that LQM-A intercalated into double-stranded DNA and damaged DNA repair.
- Subjects
METHICILLIN-resistant staphylococcus aureus; STREPTOMYCES; GRAM-positive bacteria; GRAM-negative bacteria; DNA repair; MOSAIC viruses; GENETIC translation
- Publication
Marine Drugs, 2021, Vol 19, Issue 1, p7
- ISSN
1660-3397
- Publication type
Article
- DOI
10.3390/md19010007