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- Title
The change of proinflammatory cytokine tumor necrosis factor α level in the use of meloxicam in rat model of osteoarthritis.
- Authors
Khotib, Junaidi; Utami, Naning Windi; Gani, Maria Apriliani; Ardianto, Chrismawan
- Abstract
Background: Osteoarthritis (OA) is a chronic disease in the joints. One of the proinflammatory cytokines that is thought to have a major role in the inflammatory process, the emergence of pain, and cartilage damage in OA is tumor necrosis factor α (TNF-α). Meloxicam is a nonsteroidal anti-inflammatory drug class of drugs that is relatively selective in inhibiting the activity of cyclooxygenase 2 (COX-2) formation. This study is conducted to prove the change in TNF-α level in the use of meloxicam with model in animals suffering from OA. Methods: The OA rat model was induced with sodium monoiodoacetate intra-articularly. Rats were divided into 5 groups: negative control group, positive control group, and treatment groups with various doses of meloxicam. Hyperalgesia effect was evaluated using a warm plate test, and TNF-α level was determined using enzyme-linked immunosorbent assay. Results: The treatment groups that received meloxicam at a dose of 1.0, 3.0, or 10.0 mg/kg body weight (BW) did not show significant differences in rat knee joint diameter (p = 0.99), but showed a significant difference in sensitivity to heat stimulation (p = 0.02) compared to the control group. Osteoarthritis rats experienced a significant reduction in TNF-α level after being given meloxicam at a dose of 10 mg/kg BW compared with the control group. This shows that the 10 mg/kg BW of meloxicam is a potential dose in reducing the TNF-α level in OA rat models. Conclusions: Based on these data, it can be concluded that the inhibition of pain and the development of OA by meloxicam in animal models may be assigned to a decreased level of TNF-α.
- Subjects
ACETIC acid; ANIMAL experimentation; BIOLOGICAL models; BODY weight; COMPARATIVE studies; ENZYME-linked immunosorbent assay; HEAT; HYPERALGESIA; INFLAMMATION; INTRA-articular injections; KNEE; NONSTEROIDAL anti-inflammatory agents; OSTEOARTHRITIS; PAIN; RATS; TUMOR necrosis factors; PHARMACODYNAMICS
- Publication
Journal of Basic & Clinical Physiology & Pharmacology, 2019, Vol 30, Issue 6, pN.PAG
- ISSN
0792-6855
- Publication type
Article
- DOI
10.1515/jbcpp-2019-0331