Back to matchesWe found a matchYour institution may have access to this item. Find your institution then sign in to continue.TitleOptimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.AuthorsDoench, John G; Fusi, Nicolo; Sullender, Meagan; Hegde, Mudra; Vaimberg, Emma W; Donovan, Katherine F; Smith, Ian; Tothova, Zuzana; Wilen, Craig; Orchard, Robert; Virgin, Herbert W; Listgarten, Jennifer; Root, David EPublicationNature Biotechnology, 2016, Vol 34, Issue 2, p184ISSN1087-0156Publication typeArticleDOI10.1038/nbt.3437