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- Title
Frequency of VKORC1 Gene Polymorphisms and Its Association with Warfarin Dose Requirement in Patients from Mazandaran Province.
- Authors
Salehifar, Ebrahim; Farhadi, Fahimeh; Janbabai, Ghasem; Ahangar, Nematollah
- Abstract
Background and purpose: The aim of this study was to determine the influence of three vitamin K epoxide reductase complex subunit 1 (VKORC1) polymorphisms (-1639G>A, 1173C>T and 3730G>A) on warfarin dose requirement in patients from Mazandaran province, Iran. Materials and methods: A total of 29 patients taking warfarin for at least three months with stable dose requirements enrolled in the study. DNA samples from these patients were genotyped for polymorphisms in VKORC1 gene using Real-Time PCR. Statistical analysis was performed to examine the associations between demographic characteristics (e.g. age, sex, weight, height and BMI), genetic factors, and maintenance dose of warfarin. Results: In -1639G>A polymorphism, 25 patients (86.2%) were AA, two patients (6.9%) were GA and two patients (6.9%) were GG genotype. Mean dose of warfarin in patients with AA genotype was lower but their INR was higher than other two genotypes. In 1173C>T, 26 patients (89.7%) were TT, two patients (6.9%) were CC and one patient (3.4%) was CT. The mean dose of warfarin in patients with CT genotype was also lower but their INR was higher from other two genotypes. In 3730G>A, 28 patients (96.5%) were GG and only one patient (3.5%) was GA. Significant correlation was only seen between - 1639G>A polymorphism and warfarin dose requirement. Conclusion: In the present study, we observed genetic polymorphism in all three regions. -1639G>A polymorphism was important determinants of warfarin dose requirements in our patients. The correlation between dose requirements and INR with polymorphisms was similar to other studies, however, percentage of genotypes in each polymorphism was different from other populations.
- Subjects
MAZANDARAN (Iran); VITAMIN K epoxide reductase; GENETIC polymorphisms; WARFARIN; DRUG dosage; POLYMERASE chain reaction; MEDICAL sciences
- Publication
Journal of Mazandaran University of Medical Sciences (JMUMS), 2012, Vol 22, Issue 94, p43
- ISSN
1735-9260
- Publication type
Article