We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cloning and Expression of cry11B Gene From a Local Isolate of Bacillus thuringiensis and its Mosquitocidal Activity.
- Authors
Shakoori, Farah Rauf; Zahid, Muhammad Tariq; Bukhari, Dil Ara Abbas; Shakoori, A. R.
- Abstract
The article presents a study on the expression, cloning and mosquitocidal activity of the cry11B gene from a local isolate of Bacillus thuringiensis. The researchers optimized the incubation temperature, the incubation time and the concentration of IPTG for the good expression of recombinant Cry11B protein. They conducted biotoxicity assay of crude recombinant Cry11B protein against the 3rd instar larvae of mosquito. They concluded the study with the determination of toxicity of Cry11B protein as bioinsecticide against mosquito larvae and the molecular characterization of cry11B gene from the local B.t. isolate.
- Subjects
BACILLUS thuringiensis; GENES; PROTEINS; MOSQUITO larvae; BIOLOGICAL insecticides; MICROORGANISMS
- Publication
Pakistan Journal of Zoology, 2011, Vol 43, Issue 4, p701
- ISSN
0030-9923
- Publication type
Article