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- Title
N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function.
- Authors
Xiao, Hao; Wu, Miaomiao; Shao, Fangyuan; Guan, Guiping; Huang, Bo; Tan, Bie; Yin, Yulong
- Abstract
The neonatal small intestine is susceptible to damage caused by oxidative stress. This study aimed to evaluate the protective role of antioxidant N-acetylcysteine (NAC) in intestinal epithelial cells against oxidative damage induced by H2O2. IPEC-J2 cells were cultured in DMEM-H with NAC and H2O2. After 2-day incubation, IPEC-J2 cells were collected for analysis of DNA synthesis, antioxidation capacity, mitochondrial respiration, and cell apoptosis. The results showed that H2O2 significantly decreased (P<0.05) proliferation rate, mitochondrial respiration, and antioxidation capacity and increased cell apoptosis and the abundance of associated proteins, including cytochrome C, Bcl-XL, cleaved caspase-3, and total caspase-3. NAC supplementation remarkably increased (P<0.05) proliferation rate, antioxidation capacity, and mitochondrial bioenergetics but decreased cell apoptosis. These findings indicate that NAC might rescue the intestinal injury induced by H2O2.
- Subjects
ENTEROCYTES; OXIDATIVE stress; CYSTEINE; IMMUNOMODULATORS; MITOCHONDRIAL physiology; EPITHELIAL cells; ANTIOXIDANTS
- Publication
Mediators of Inflammation, 2016, p1
- ISSN
0962-9351
- Publication type
Article
- DOI
10.1155/2016/8364279