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- Title
Expression of genes involved in key metabolic processes during winter flounder ( Pseudopleuronectes americanus) metamorphosis.
- Authors
Vagner, Marie; de Montgolfier, Benjamin; Sévigny, Jean-Marie; Tremblay, Réjean; Audet, Céline
- Abstract
The aim of this study was to better understand the molecular events governing ontogeny in winter flounder ( Pseudopleuronectes americanus (Walbaum, 1792)). The expression of seven genes involved in key metabolic processes during metamorphosis were measured at settlement (S0), at 15 (S15), and 30 (S30) days after settlement and compared with those in pelagic larvae prior to settlement (PL). Two critical stages were identified: (1) larval transit from the pelagic to the benthic habitat (from PL to S0) and (2) metamorphosis maturation, when the larvae stay settled without growth (from S0 to S30). Growth hormone ( gh) gene expression significantly increased at S0. At S30, an increase in cytochrome oxidase ( cox) gene expression occurred with a second surge of gh gene expression, suggesting that enhanced aerobic capacity was supporting growth before the temperature decrease in the fall. Expression patterns of pyruvate kinase ( pk), glucose-6-phosphate dehydrogenase ( g6pd), and bile salt activated lipase ( bal) genes indicated that energy synthesis may be mainly supplied through glycolysis in PL, through the pentose-phosphate pathway at settlement, and through lipid metabolism at S30. The expression of the heat-shock protein 70 ( hsp70), superoxide dismutase ( sod), cox, and peroxiredoxin-6 ( prx6) genes revealed that oxidative stress and the consequent development of antioxidative protection were limited during the PL stage, reinforced at settlement, and very high at S30, certainly owing to the higher growth rate observed at this period.
- Subjects
METAMORPHOSIS; SOMATOTROPIN; ANTIOXIDANTS; ENERGY metabolism; WINTER flounder
- Publication
Canadian Journal of Zoology, 2013, Vol 91, Issue 3, p156
- ISSN
0008-4301
- Publication type
Article
- DOI
10.1139/cjz-2012-0240