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- Title
Role of Accessory Cells in the Activation of Pure T Cells via the T Cell Receptor-CD3 Complex or with Phytohaemagglutinin.
- Authors
Halvorsen, R.; Leivestad, T.; Gaudernack, G.; Thorsby, E.
- Abstract
The ability of different subpopulations of blood mononuclear cells to serve as accessory cells in the activation of CD4+ and CD8+ T cells via Ti-CD3 or with phytohaemagglutinin (PHA) was studied. Pure CD4+ or CD8+ T cells did not respond to particle-bound anti-CD3 monoclonal antibodies (MoAb) or PHA, whereas responses were seen when non-T cells served as accessory cells. Removal of class II-positive cells from peripheral blood mononuclear cells (PBMC) or from non-T cells diminished, but did not completely abolish, the responses in both T cell subsets, indicating that the accessory cells are mainly found among the class II-positive cells. However, the class II molecules themselves were not involved, as demonstrated in antibody- blocking experiments. Removal of monocytes decreased the ability of non-T cells to serve as accessory cells for both CD4+ and CD8+ cells in PHA activation. In contrast, the removal of monocytes resulted in an enhanced activation by anti-CD3 MoAb in CD4+ T cell, while the activation of CD8+ T cells was less affected. Positively selected B cells were effective accessory cells in anti-CD3 and PHA activation. Furthermore, Epstein-Barr virus (EBV)-transformed B cell lines were very potent accessory cells both in anti-CD3 and PHA activation of P cells, and showed the strongest accessory cell function observed in this system on a per cell basis.
- Subjects
T cells; PHYTOHEMAGGLUTININS; CD antigens; MONOCLONAL antibodies; B cells; EPSTEIN-Barr virus; MONOCYTES
- Publication
Scandinavian Journal of Immunology, 1988, Vol 27, Issue 5, p555
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1988.tb02383.x