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- Title
薏仁麩皮活性萃取物製備程序最適化條件及指標成分定性分析.
- Authors
鍾 成 沛; 李 明 怡; 陳 思 綺; 林 弘 育; 江 文 章; 夏 詩 閔
- Abstract
Adlay bran (AB) possesses physiological functions on inhibiting cancer, anti-inflammation, improving metabolic syndromes, and modulation on endocrine. Phenolic compounds, polymethoxyflavones (PMFs), and unique coixspirolactams were regarded as important active components in AB. However, optimized extracting procedure for above ingredients is lacking. In the present study, yield, total polyphenol, and total flavonoids contents in AB ethanolic extract (ABE) were founded to be affected mainly by temperature and concentration of ethanol(aq). According to response surface methodology (RSM) evaluation, the optimized extracting condition of ABE was under 50°C with 8-folds of 55% ethanol(aq) for 120 min. ABE made by above procedures was analyzed on a high-performance liquid chromatography (HPLC) system; and p-hydroxybenzoic acid (11.86 μg/g), vanillin (6.57 μg/g), and ferulic acid (6.36 μg/g) were relatively abundant in ABE. Moreover, coixspirolactam E and PMFs, including 3,5,7,4'-tetramethoxyflavone, 3,5,7,3',4'-pentamethoxyflavone, 3,5,6,7,8,3',4'-heptamethoxyflavone, tangererin, and nobiletin, were clearly identified by HPLC-mass spectrum (HPLC-MS) platform. The results would be used for product development and quality control of ABE.
- Publication
Taiwanese Journal of Agricultural Chemistry & Food Science, 2023, Vol 61, Issue 3, p153
- ISSN
1605-2471
- Publication type
Article