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- Title
Primary Study on Antibiotic and Anticancer Secondary Metabolites and Their Potentially Related Genes from Streptomyces vietnamensis.
- Authors
DENG Ming-rong; GUO Jun; ZHU Hong-hui
- Abstract
Streptomyces vietnamensis is a newly designated species, possessing the ability to produce diffusible violet-blue pigment on a variety of media. The study on the bioactivities of its secondary metabolites was carried out. The ethyl acetate extracts of fermentation broth exhibited high antibacterial activity against all the tested Gram-positive bacteria, and it also showed antibacterial effect against some strains of Gram-negative bacteria to different extents. Two blue pigments (B1, B2) in the extract content are the main antibacterial compounds as revealed by TLC-bioautography. The crude extracts at a concentration of 20 µg/mL could inhibit the growth of HeLa cells by a rate of 96.7% after treatment for 48 h, showing excellent anticancer activity. Four PKS/NRPS-related sequences were obtained by PCR using degenerate primers, two of which showed high homology with PKS genes responsible for the biosynthesis of several known antibiotics, suggesting they might be the important pathways for biosynthesis of bioactive secondary metabolites in S. vietnamensis. These sequences could be used as gene sources for combinatorial biosynthesis.
- Subjects
STREPTOMYCES; ANTIBIOTICS; ANTINEOPLASTIC agents; METABOLITES; GRAM-positive bacteria
- Publication
Natural Product Research & Development, 2010, Vol 22, Issue 3, p367
- ISSN
1001-6880
- Publication type
Article