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- Title
Gene Profiles During Vestibular Compensation in Rats After Unilateral Labyrinthectomy.
- Authors
Moo Kyun Park; Byung Don Lee; Jong Dae Lee; Hak Hyun Jung; Sung Won Chae
- Abstract
Objectives: The aims of this study were to determine changes in gene expression in the chronic state of vestibular compensation by microarray analysis and to validate the asymmetrical levels of gene expression in the ipsilateral and contralateral vestibular nucleus complexes (VNCs). Methods: Microarray analysis was used to examine the expression of genes up-regulated or down-regulated in the ipsilateral VNC at 1 and 7 days after unilateral labyrinthectomy. Up-regulated or down-regulated gene expression in the ipsilateral and contralateral VNCs was then validated by reverse transcriptase polymerase chain reaction at 1,7, 14, and 28 days after labyrinthectomy. Results: The genes down-regulated at 1 day after labyrinthectomy and up-regulated at 7 days after labyrinthectomy as determined by microarray analysis and reverse transcriptase polymerase chain reaction were zinc finger protein 307, zinc metallopeptidase, P34, calcitonin receptor, insulin-like growth factor binding protein 5, GATA binding protein 3, and CD 151. Expression of zinc finger protein 307, zinc metallopeptidase, P34, and calcitonin receptor was up-regulated even after 7 days in the contralateral VNC of rats that had labyrinthectomy. Conclusions: This study demonstrated changes in gene expression in rats during the chronic phase of vestibular compensation after unilateral labyrinthectomy and provided profiles of these changes in gene expression.
- Subjects
VESTIBULAR apparatus; VESTIBULAR apparatus diseases; ANIMAL experimentation; BRAIN stem; GENE expression; POLYMERASE chain reaction; RATS; STATISTICS; DATA analysis; REVERSE transcriptase polymerase chain reaction; DATA analysis software; MICROARRAY technology; DESCRIPTIVE statistics; GENETICS; SURGERY
- Publication
Annals of Otology, Rhinology & Laryngology, 2012, Vol 121, Issue 11, p761
- ISSN
0003-4894
- Publication type
Article
- DOI
10.1177/000348941212101110