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- Title
Application of Statistical Analysis for the Optimization of Mycelia and Polysaccharide Production by Tremella aurantialba.
- Authors
Zhicai Zhang; Yin Li; Kechang Zhang
- Abstract
Statistical analyses were applied to optimize the medium composition for the mycelial growth and polysaccharide production by Tremella aurantialba in shake flask cultures. Firstly; four significant factors (xylan, peptone, wheat bran and corn powder) on mycelial growth and polysaccharide yield (p≤0.05) were obtained using one-at-a-time design. Subsequently, in order to study the mutual interactions between variables, the effects of these factors were further investigated using four-factor, three-level orthogonal test design and the optimal composition was (in g/L): xylan 40, peptone 10, wheat bran 20, corn powder 20, KH2PO4 1.2 and MgSO4·7H2O 0.6. Finally, the maximum mycelium yield and polysaccharide production in 50-litre stirred-tank bioreactor reached 36.8 and 3.01 g/L under the optimized medium, respectively.
- Subjects
TREMELLA; MYCELIUM; XYLANS; POLYSACCHARIDES; STATISTICS; PEPTONES; BRAN; POWDERS
- Publication
Food Technology & Biotechnology, 2007, Vol 45, Issue 1, p45
- ISSN
1330-9862
- Publication type
Article